abstract：:Rabbit skeletal muscle sarcoplasmic reticulum (SR) was fractionated by isopycnic density gradient centrifugation into longitudinal tubules (LSR) and terminal cisternae (TC). Junctional face membranes (JFM) were obtained by Triton X-100 treatment of the TC fraction (Costello, B., Chadwick, C., Saito, A., Chu, A., Maure...

journal_title：Archives of biochemistry and biophysics

authors： Volpe P,Gutweniger HE,Montecucco C

更新日期：1987-02-15 00:00:00

abstract：:Differential light absorption spectra of the bilirubin-albumin 1:1 complex, obtained on addition of 20 different sulfonamides, differ with respect to shape and amplitude. This finding seems to indicate that the sulfonamide molecule is bound in direct touch with the bilirubin. The light absorption spectrum of bilirubin...

journal_title：Archives of biochemistry and biophysics

authors： Brodersen R,Knudsen A,Pedersen AO

更新日期：1987-02-01 00:00:00

abstract：:In the present study, the hepatic microsomal and peroxisomal bifunctional trans-2-enoyl CoA hydratases were isolated and purified from rats treated with 2% di-(2-ethylhexyl)phthalate for 8 days. These two enzymes (microsomal and peroxisomal) were purified with the identical purification procedures and had identical mo...

journal_title：Archives of biochemistry and biophysics

authors： Cook L,Ghesquier D,Nagi MN,Favreau LV,Cinti DL

更新日期：1987-02-01 00:00:00

abstract：:The metabolic fates of NG,NG-and NG,N'G-dimethylarginines in rats were investigated isotopically and novel metabolites, alpha-keto-delta-(N,N-dimethylguanidino)-and alpha-keto-delta-(N,N'-dimethylguanidino)valeric acids and gamma-(N,N-dimethylguanidino)-and gamma-(N,N'-dimethylguanidino)butyric acids were identified. ...

journal_title：Archives of biochemistry and biophysics

authors： Ogawa T,Kimoto M,Watanabe H,Sasaoka K

更新日期：1987-02-01 00:00:00

abstract：:Stimulation of pig peripheral blood lymphocytes with concanavalin A (Con A) provoked a rapid increase (two- to threefold) in the rate of ouabain-inhibitable K+ uptake observable within 3-10 min of stimulation with mitogen. At least two phases can be distinguished in the activation of the Na+/K+ pump: the early phase (...

journal_title：Archives of biochemistry and biophysics

authors： Prasad KV,Severini A,Kaplan JG

更新日期：1987-02-01 00:00:00

abstract：:Diesters of 3-hydroxy C8, C10, and C12 acids, the female mallard duck pheromones, were found as the major products of the uropygial glands only during the breeding season. The 3-hydroxy acids were identified by mass spectrometry of the trimethylsilyl ethers of the methyl esters and of the diols derived from LiAlH4 red...

journal_title：Archives of biochemistry and biophysics

authors： Kolattukudy PE,Rogers L

更新日期：1987-01-01 00:00:00

abstract：:In the absence of NADPH, the addition of an arachidonic acid hydroperoxide, 15-hydroperoxyeicosa-5,8,11,13-tetraenoic acid, to liver microsomes, prepared from phenobarbital-treated rats, resulted in the formation of two major metabolites and several minor products, some of which have been purified by reverse-phase hig...

journal_title：Archives of biochemistry and biophysics

authors： Weiss RH,Arnold JL,Estabrook RW

更新日期：1987-01-01 00:00:00

abstract：:The activity of acetyl-CoA carboxylase (ACC), the rate-limiting enzyme of fatty acid biosynthesis, can be regulated by both adenine and guanine nucleotides in vitro. We have employed two inhibitors of IMP dehydrogenase, ribavarin and tiazofurin, to investigate a possible role for intracellular nucleotides in ACC regul...

journal_title：Archives of biochemistry and biophysics

authors： Witters LA,Mendel DB,Colliton JW

更新日期：1987-01-01 00:00:00

abstract：:The nonenzymatic reaction of the cytotoxic compounds menadione (2-methyl-1,4-naphthoquinone) and 1,4-naphthoquinone (a reactive metabolite of 1-naphthol) with reducing agents such as NADPH and glutathione led to the formation of semiquinone-free radicals, which were detected with electron spin resonance spectroscopy. ...

journal_title：Archives of biochemistry and biophysics

authors： Takahashi N,Schreiber J,Fischer V,Mason RP

更新日期：1987-01-01 00:00:00

abstract：:The soluble enzymatic luminescent system of the dinoflagellate Pyrocystis lunula (luciferase-luciferin) is coupled with an enzymatic NAD(P)H-dependent reaction. The enzyme is a soluble reductase (Mr 47,000) which catalyzes, in the presence of NAD(P)H, the reduction of a molecule called P630. Reduced P630 has the same ...

journal_title：Archives of biochemistry and biophysics

authors： Fresneau C,Hill M,Lescure N,Arrio B,Dupaix A,Volfin P

更新日期：1986-12-01 00:00:00

abstract：:A procedure was developed for the purification of an acetone-inducible form of cytochrome P-450 (P-450ac) to electrophoretical homogeneity from liver microsomes of acetone-treated rats. The P-450ac preparation containing 16.0 to 16.5 nmol P-450/mg protein moved as a single protein band with an estimated molecular weig...

journal_title：Archives of biochemistry and biophysics

authors： Patten CJ,Ning SM,Lu AY,Yang CS

更新日期：1986-12-01 00:00:00

abstract：:Conversion of geranyl pyrophosphate to cyclic monoterpenes is considered to involve the preliminary isomerization of this acyclic precursor to enzyme-bound linalyl pyrophosphate and the cyclization of this tertiary intermediate. 2-Fluorogeranyl pyrophosphate and 2-fluorolinalyl pyrophosphate are effective competitive ...

journal_title：Archives of biochemistry and biophysics

authors： Croteau R

更新日期：1986-12-01 00:00:00

abstract：:We have isolated an activator of collagenase from medium conditioned with articular cartilage. The activity is contained in an acidic protein appearing as a doublet band of Mr 57,000 and 56,000 on sodium dodecyl sulfate polyacrylamide gels. Both components of the doublet have identical isoelectric points as demonstrat...

journal_title：Archives of biochemistry and biophysics

authors： Treadwell BV,Neidel J,Pavia M,Towle CA,Trice ME,Mankin HJ

更新日期：1986-12-01 00:00:00

abstract：:Activation of different forms of adenylate cyclases (AC) by forskolin and displacement of [14,15-3H]dihydroforskolin binding from membranes by forskolin in the absence or presence of specific stimulatory hormone and beta, gamma-imidoguanosine 5'-triphosphate (Gpp(NH)p) have been studied. These conditions have been use...

journal_title：Archives of biochemistry and biophysics

authors： Shi QH,Ruiz JA,Ho RJ

更新日期：1986-11-15 00:00:00

abstract：:Aldehyde oxidase (EC 1.2.3.1) plays a role in the oxidation of aromatic heterocyclic compounds ingested by some higher vertebrates. To better understand this function, the specificity of the rabbit liver enzyme toward purines and their analogs was quantitatively studied. The chemical nature of the 6-substituent of pur...

journal_title：Archives of biochemistry and biophysics

authors： Hall WW,Krenitsky TA

更新日期：1986-11-15 00:00:00

abstract：:Heme-catalyzed decomposition of unsaturated hydroperoxy fatty acids has been proposed to proceed via carbon-centered free radicals (delocalized at positions C11, C12, and C13 for 15-hydroperoxy-eicosatetraenoic acid (15-HPETE). The stable products are usually epoxy fatty acids and epoxy alcohols. Hydroperoxides from a...

journal_title：Archives of biochemistry and biophysics

authors： Schreiber J,Mason RP,Eling TE

更新日期：1986-11-15 00:00:00

abstract：:Three iodinated derivatives of ubiquitin have been synthesized and these derivatives have been characterized in the ubiquitin-dependent protein degradation system. With chloramine-T as the oxidant, a derivative containing monoiodotyrosine is formed in the presence of 1 M KI and a derivative containing diiodotyrosine i...

journal_title：Archives of biochemistry and biophysics

authors： Cox MJ,Haas AL,Wilkinson KD

更新日期：1986-11-01 00:00:00

abstract：:The role of chalcone synthase in the regulation of flavonoid biosynthesis during organogenesis of oat primary leaves has been investigated at the level of enzyme activity and mRNA translation in vitro. Chalcone synthase was purified about 500-fold. The apparent Km values were 1.5 and 6.3 microM for 4-coumaroyl-CoA and...

journal_title：Archives of biochemistry and biophysics

authors： Knogge W,Schmelzer E,Weissenböck G

更新日期：1986-11-01 00:00:00

abstract：:Casein kinase 1 phosphorylated rabbit skeletal muscle glycogen synthase at both seryl and threonyl residues. With glycogen synthase phosphorylated up to 7.5 mol phosphate/mol subunit, about 26% of the phosphate was present in the N-terminal cyanogen bromide fragment (CB1) and 74% in the C-terminal fragment (CB2). Both...

journal_title：Archives of biochemistry and biophysics

authors： Guasch MD,Plana M,Vila J,Itarte E

更新日期：1986-11-01 00:00:00

abstract：:Maturation of the precursor forms of bovine cholesterol side-chain cleavage cytochrome P-450 (P-450SCC) and 11 beta-hydroxylase cytochrome P-450 (P-450(11)beta) was investigated using mitochondria from bovine corpus luteum. The results show that both precursors, whose synthesis was directed by bovine adrenocortical RN...

journal_title：Archives of biochemistry and biophysics

authors： Matocha MF,Waterman MR

更新日期：1986-11-01 00:00:00

abstract：:Artemia trypsin-like proteinase has been reported previously to be highly inhibited in the embryo (B. Ezquieta and C.G. Vallejo (1985) Comp. Biochem. Physiol. 82B, 731-736). We report now that Artemia lipovitellin, the major storage protein complex, inhibits the proteinase. We have carried out an in vitro study of the...

journal_title：Archives of biochemistry and biophysics

authors： Ezquieta B,Vallejo CG

更新日期：1986-11-01 00:00:00

abstract：:Sensitive assays were developed for long chain and very long chain fatty acid oxidation in human skin fibroblast homogenates. Stearic and lignoceric acids were degraded by the fibroblasts by the beta-oxidation pathway. The cofactor requirements for stearic and lignoceric acid beta-oxidation were very similar but not i...

journal_title：Archives of biochemistry and biophysics

authors： Singh H,Poulos A

更新日期：1986-10-01 00:00:00

abstract：:An in vitro acrosome-like reaction was induced in spermatozoa from the boar cauda epididymis by incubation in Tyrode's solution containing 1 mg/ml fatty acid-free bovine serum albumin. Plasma membranes were isolated from the spermatozoa at different times during the incubation and analyzed for their lipid composition....

journal_title：Archives of biochemistry and biophysics

authors： Nikolopoulou M,Soucek DA,Vary JC

更新日期：1986-10-01 00:00:00

abstract：:Rat liver microsomes catalyze a vanadate-stimulated oxidation of NAD(P)H, which is augmented by paraquat and suppressed by superoxide dismutase, but not by catalase. NADPH oxidation was a linear function of the concentration of microsomes in the absence of vanadate, but was a saturating function in the presence of van...

journal_title：Archives of biochemistry and biophysics

authors： Liochev S,Fridovich I

更新日期：1986-10-01 00:00:00

abstract：:The mitochondrial ATP synthase/ATPase (F0F1 ATPase) is perhaps the most complex enzyme known. In animal systems it consists of a minimum of 11 different polypeptide chains, 10 (or more) of which appear to be essential for function, and 1 called the "ATPase inhibitor peptide" which is involved in regulation. Recent stu...

journal_title：Archives of biochemistry and biophysics

authors： Schwerzmann K,Pedersen PL

更新日期：1986-10-01 00:00:00

abstract：:Isolated adrenocortical carcinoma cells of rat contain alpha 2- and beta-adrenergic receptors. When these cells are incubated with alpha 2-adrenergic agonists, there is a concentration-dependent increase of cyclic GMP that is blocked by the alpha 2-adrenergic antagonist yohimbine but not by the beta-antagonist propran...

journal_title：Archives of biochemistry and biophysics

authors： Jaiswal N,Sharma RK

更新日期：1986-09-01 00:00:00

abstract：:A protocol for dry weight determination of the concentration of protein, using 0.2-1.0 mg of protein per sample, has been presented and applied to nine proteins: bovine serum albumin, ovalbumin, bovine carbonic anhydrase B, galactoside binding protein (rabbit), lens calinaris lectin B, green pea lectin, soy bean agglu...

journal_title：Archives of biochemistry and biophysics

authors： Nozaki Y

更新日期：1986-09-01 00:00:00

abstract：:Photoinduced reduction of methylviologen (MV2+) by ethylenediaminetetraacetate (EDTA3-), which was sensitized by thiacarbocyanine dyes having long alkyl chains (C+m-n) embedded in palmitoyl lysophosphatidylcholine micelle and dipalmitoyl phosphatidylcholine liposomal membrane, was carried out. The formation rate of re...

journal_title：Archives of biochemistry and biophysics

authors： Nakagaki M,Komatsu H,Handa T

更新日期：1986-09-01 00:00:00

abstract：:The effect of CAM [cyclic AMP, Mg-ATP, and 3-isobutyl, 1-methylxanthine (MIX)] on triacylglycerol (TG) lipase activity in extracts from heparin-perfused rat heart was determined. TG lipase activity in homogenate, 10,000g supernatant, 105,000g supernatant, ammonium sulfate supernatant, and the eluate from heparin-Sepha...

journal_title：Archives of biochemistry and biophysics

authors： Palmer WK,Caruso RA,Oscai LB

更新日期：1986-09-01 00:00:00

abstract：:A biochemical basis for the pea and lentil lectin resistance of two Chinese hamster ovary (CHO) cell mutants, Lec13 and Lec13A, was investigated. Studies of the G glycopeptides of vesicular stomatitis virus grown in the mutants indicated that Lec13 cells essentially lack the ability to add fucose to complex carbohydra...

journal_title：Archives of biochemistry and biophysics

authors： Ripka J,Adamany A,Stanley P

更新日期：1986-09-01 00:00:00

abstract：:The polyisoprenyl phosphate dephosphorylating activity of rat liver has been investigated with regard to substrate specificity, subcellular distribution, and transmembrane orientation. Total liver microsomes were employed as a source of enzymatic activity against a variety of 32P-labeled substrates. Susceptibility to ...

journal_title：Archives of biochemistry and biophysics

authors： Keller RK,Adair WL Jr,Cafmeyer N,Simion FA,Fleischer B,Fleischer S

更新日期：1986-08-15 00:00:00

abstract：:NADP-malate dehydrogenase activity, the ratio of NADPH to NADP, and thioredoxin redox state in Zea mays chloroplasts were determined after various treatments. Following transfer from dark to light, NADP-malate dehydrogenase was activated more than 20-fold within 10 min while the proportion of pyridine nucleotide as NA...

journal_title：Archives of biochemistry and biophysics

authors： Rebeille F,Hatch MD

更新日期：1986-08-15 00:00:00

abstract：:A10 smooth muscle cells, derived from embryonic rat thoracic aorta, responded to the atrial natriuretic factor (ANF) with increased levels of cyclic GMP. These cells possess high-affinity (apparent Kd = 50 pM) plasma membrane receptors for ANF. Internalization of ANF at 37 degrees C was indicated by the following: app...

journal_title：Archives of biochemistry and biophysics

authors： Napier MA,Arcuri KE,Vandlen RL

更新日期：1986-08-01 00:00:00

abstract：:The keto form of oxaloacetate (OAA), a product of phosphoenolpyruvate carboxylase (PEPC) activity, can undergo various nonenzymatic conversions which make conventional methods of assaying the enzyme difficult, because the products may either act as inhibitors or go undetected. In studies with PEPC isolated from leaves...

journal_title：Archives of biochemistry and biophysics

authors： Walker GH,Ku MS,Edwards GE

更新日期：1986-08-01 00:00:00

abstract：:The treatment of male rats with Hg2+ resulted in significant alterations in heme and hemoprotein metabolism in the adrenal gland which, in turn, were reflected in abnormal steroidogenic activities and steroid output. Twenty-four hours after the administration of 30 mumol of HgCl2/kg (sc) the mitochondrial heme and cyt...

journal_title：Archives of biochemistry and biophysics

authors： Veltman JC,Maines MD

更新日期：1986-08-01 00:00:00

abstract：:We have previously shown that rat hepatic cytochromes P450f, P450g, P450h, and P450i possess a high degree of immunochemical and, presumably, structural relatedness. Polyclonal antibodies directed against cytochromes P450f and P450g were made monospecific by immunoabsorption against the cross-reactive proteins. The sp...

journal_title：Archives of biochemistry and biophysics

authors： Bandiera S,Ryan DE,Levin W,Thomas PE

更新日期：1986-08-01 00:00:00

abstract：:A cytoplasmic 10S ribonucleoprotein particle (iRNP), which is isolated from chick embryonic muscle, is a potent inhibitor of mRNA translation in vitro and contains a 4S translation inhibitory RNA species (iRNA). The iRNP particle shows similarity in size to the small nuclear ribonucleoprotein (snRNP) particles. Certai...

journal_title：Archives of biochemistry and biophysics

authors： Boak AM,Kovacs SA,Agris PF,Chakraborty D,Sarkar S

更新日期：1986-07-01 00:00:00

abstract：:Spontaneously beating isolated rat atria were subjected to 1 h of anoxia at 37 degrees C in various cardioplegic solutions. Contraction continued for different times upon initiation of anoxia, depending on the nature of the cardioplegic solution. Two hundred micromolar P1,P5-di(adenosine-5')pentaphosphate (Ap5A) stopp...

journal_title：Archives of biochemistry and biophysics

authors： Savabi F,Bessman SP

更新日期：1986-07-01 00:00:00

abstract：:Synephrinase, an enzyme catalyzing the conversion of (-)-synephrine into p-hydroxyphenylacetaldehyde and methylamine, was purified to apparent homogeneity from the cell-free extracts of Arthrobacter synephrinum grown on (+/-)-synephrine as the sole source of carbon and nitrogen. A 40-fold purification was sufficient t...

journal_title：Archives of biochemistry and biophysics

authors： Manne V,Kutty KR,Pillarisetti SR

更新日期：1986-07-01 00:00:00

abstract：:Horseradish peroxidase (EC 1.11.1.7) is shown to catalyze the aerobic oxidation of lysozyme, bovine serum albumin, and protamine adducts with glycolaldehyde at physiological pH. This reaction is accompanied by light emission, which is attributed to the generation of triplet species. The intensity of chemiluminescence ...

journal_title：Archives of biochemistry and biophysics

authors： de Medeiros MH,Bechara EJ

更新日期：1986-07-01 00:00:00